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β-tubulin Mouse Monoclonal Antibody(Mix-mA™)

2015-03-30 14:13:03 点击数:
关键字: XHY167

 

β-tubulin Mouse Monoclonal Antibody(Mix-mA™)

Catalog

XHY167M

XHY167L

Quantity

50µL

100µL

For research use only.

 

Application: WB, IHC    

Species Cross-Reactivity: H, R, M, Mk, Dg, C, Hm, Rb, Sh   

Molecular Weight: 55KD

Isotype: IgG1

 

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000-10,000 IHC: 1:200  IF: 1:200

Optimal dilutions should be determined by the end user.

 

Specificity: Antibody can detects endogenous β-tubulin protein.

Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the

cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da.

Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be

noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in

adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

 

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation   ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep

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