For research use only.

Application: WB, IHC, IF   

Species Cross-Reactivity: H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh

Molecular Weight: 43KD

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000-10,000 IHC: 1:200  IF: 1:200

Optimal dilutions should be determined by the end user.

Specificity: Antibody can detects endogenous β-actin protein..

Background: β-actin is one of six different actin isoforms that have been identified. The actin molecules

found in cells of various species and tissues tend to be very similar in their immunological and physical

properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting.

However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression

of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these

tissues..

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                       ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep