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β-tubulin Mouse Monoclonal Antibody(M7)

2014-09-24 11:18:08 点击数:
关键字: XHY163

 

β-tubulin Mouse Monoclonal Antibody(M7)

Catalog

XHY163M

XHY163L

Quantity

50µL

100µL

For research use only.

 

Application: WB  

Species Cross-Reactivity: H, R, M    

Molecular Weight: 55KD

Isotype: IgG1

 

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000-10,000

Optimal dilutions should be determined by the end user.

 

Specificity: The β -tubulin antibody can detects endogenous β-tubulin protein.

Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the

cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da.

Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be

noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in

adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

 

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                          ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep

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