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β-tubulin Mouse Monoclonal Antibody(M7) |
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Catalog |
XHY163M |
XHY163L |
Quantity |
50µL |
100µL |
For research use only.
Application: WB
Species Cross-Reactivity: H, R, M
Molecular Weight: 55KD
Isotype: IgG1
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Store : -20°C. Do not aliquot the antibody.
Recommended dilutions: WB: 1:5,000-10,000
Optimal dilutions should be determined by the end user.
Specificity: The β -tubulin antibody can detects endogenous β-tubulin protein.
Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the
cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da.
Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be
noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in
adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
Applications:WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep