For research use only.

Application: WB  

Species Cross-Reactivity: H 

Molecular Weight: 116KD(Pro) /89(Active)

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:1,000-3,000

Optimal dilutions should be determined by the end user.

Specificity: Antibody can detects endogenous pro and active PARP protein.

Background: Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD + ADP-ribosyltransferase 1 or

poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in

repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or

inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when

these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA

(dsDNA) breaks accumulate.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                        ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep