For research use only.

Application: WB, IP, IF

Species Cross-Reactivity: N/A   

Molecular Weight: N/A 

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000-10,000 IP: 1:200  IF: 1:500

Optimal dilutions should be determined by the end user.

Specificity: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.

Background: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His)

residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can

greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify,

detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                      ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep