For research use only.

Application: WB, IHC, IF

Species Cross-Reactivity: Zebrafish

Molecular Weight: 55KD

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000 -20,000 IHC: 1:200  IF: 1:200

Optimal dilutions should be determined by the end user.

Specificity: The β-tubulin antibody can detects Zebrafish endogenousβ-tubulin protein. 

Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of

the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about

55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However

it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of

β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for

these tissues..

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                             ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep