For research use only.

Applications: WB, IHC  

Species Cross-Reactivity: H,M,R    

Molecular Weight: ~65KD     

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:1,000-3,000  IHC: 1:200

Optimal dilutions should be determined by the end user.

Specificity: NFkB p65  Mouse monoclonal antibody detects endogenous p65 proteins 

Background: NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for

termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by

CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and

impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a

pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of

immune function required for resistance to infection..

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                           ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep