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β -tubulin Mouse Monoclonal Antibody(5G3)

2014-07-23 19:27:21 点击数:
关键字: XHY043

 

β -tubulin Mouse Monoclonal Antibody(5G3)

Catalog

XHY043M

XHY043L

Quantity

50µL

100µL

For research use only.

 

Applications: WB, IHC

Species Cross-Reactivity: H, R, M, Mk, Dg, C, Hm, Rb, Sh

Molecular Weight: 55KD

Isotype: IgG1

 

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1: 5,000, IHC: 1:200

Optimal dilutions should be determined by the end user.

 

Specificity: Antibody can detects endogenous β-tubulin protein 

Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the

cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da.

Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted

that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue

is very low and therefore β-Tubulin should not be used as loading control for these tissues.

 

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                        ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep

 

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