For research use only.

Applications: WB, IP, IF

Species Cross-Reactivity: N/A

Molecular Weight: N/A

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000   IP:1:200  IF:1:1,000

Optimal dilutions should be determined by the end user.

Specificity: The VSV-G tag antibody can recognize C-terminal, internal, and N-terminal VSV-G fusion proteins.

Background: Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released

from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of

the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be

used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as

sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral

components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose

cushion ultracentrifugation.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation  

                       ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep